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使用 PRIMO 微模式系统对蛋白质的光诱导分子吸附,研究细胞对细胞外基质蛋白的反应
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins
Please note that all translations are automatically generated.
Click here for the English version.
使用 PRIMO 微模式系统对蛋白质的光诱导分子吸附,研究细胞对细胞外基质蛋白的反应
DOI:
10.3791/60092-v
•
09:49 min
•
October 11, 2019
•
Cristina Melero*
1
,
Aljona Kolmogorova*
1
,
Paul Atherton
,
Brian Derby
,
Adam Reid
4
,
Karin Jansen
,
Christoph Ballestrem
1
Faculty of Biology, Medicine and Health. Division of Cell Matrix, Biology and Regenerative Medicine, Wellcome Trust Centre for Cell-Matrix Research
,
The University of Manchester
,
2
School of Materials
,
The University of Manchester
,
3
Blond McIndoe Laboratories, School of Biological Sciences, Faculty of Biology, Medicine and Health, Division of Cell Matrix Biology and Regenerative Medicine, The University of Manchester
,
Manchester Academic Health Science Centre
,
4
Department of Plastic Surgery & Burns, Wythenshawe Hospital, Manchester University NHS Foundation Trust
,
Manchester Academic Health Science Centre
,
5
Department of Pathology
,
UMC Utrecht
Chapters
00:04
Title
00:57
Design of Pattern Templates and Plasma Cleaning
01:52
Passivation with PLL-PEG
02:54
System Calibration
04:36
Software Set-up and Photopatterning
05:29
Protein Incubation and Blocking Unspecific Binding Sites
06:43
Second Round of Photopatterning and Protein Incubation
07:39
Results: Micropatterning for the Study of Cell Responses to Extracellular Matrix Proteins
09:03
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们的总体目标是了解细胞如何感知细胞外线索,导致定向的斧头生长。在这里,我们描述了光诱导分子吸附蛋白质的方法,用于生成细胞外基质成分的定义的微模式,以研究控制斧头生长和路径发现的特定事件。
Tags
Micro-patterning
LIMAP
Cell Response
Extracellular Matrix
Protein Adsorption
Nerve Repair
Regenerative Medicine
Cell Biology
Cell Proliferation
Cell Differentiation
Cell Migration
PDMS Stencils
PLL-PEG
Photoinitiator
PRIMO System
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