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Neuroscience

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In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes
 

In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes

Article DOI: 10.3791/57869 08:17 min September 26th, 2018
September 26th, 2018

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Summary

In vivo microdialysis has enabled collection of molecules present in brain interstitial fluid (ISF) from awake, freely-behaving animals. In order to analyze relatively large molecules in ISF, the current article specifically focuses on the microdialysis protocol using probes with high molecular weight cut off membranes.

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In Vivo Microdialysis Method Collect Large Extracellular Proteins Brain Interstitial Fluid High-molecular Weight Cut-off Probes Neuroscience Research Signal Transduction Substrate Transport Waste Clearance Interstitial Fluid (ISF) Awake Freely-moving Animals Skull Anesthetized Mouse Ear Bars Nose Clamp Adapter Ointment Stereotaxic Apparatus Scalpel Skin Incision Drill Manipulator AP Coordinate ML Coordinate Bregma Coordinate Vertical Coordinate Burr Hole Parietal Bone
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