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In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes
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JoVE Journal Neuroscience
In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes

In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes

DOI:
10.3791/57869-v

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08:17 min

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September 26, 2018

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1Department of Neuropathology, Graduate School of Medicine,University of Tokyo

Chapters

  • 00:04Title
  • 00:42Guide Cannula Implantation
  • 04:17Microdialysis Setup
  • 07:24Results: Representative Interstitial Fluid (ISF) Tau Changes Upon Picrotoxin Reverse Microdialysis
  • 07:52Conclusion

Summary

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Automatic Translation

In vivo microdialysis has enabled collection of molecules present in brain interstitial fluid (ISF) from awake, freely-behaving animals. In order to analyze relatively large molecules in ISF, the current article specifically focuses on the microdialysis protocol using probes with high molecular weight cut off membranes.

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Keywords: In Vivo MicrodialysisBrain Interstitial FluidHigh-molecular Weight Cut-off ProbesNeuroscience ResearchSignal TransductionSubstrate TransportWaste ClearanceExtracellular MoleculesAwake Freely-moving AnimalsStereotaxic ApparatusGuide CannulaDental CementMicrodialysis Probe

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