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Lys-indusert molekylær absorpsjon av proteiner ved hjelp av PRIMO system for mikro-mønstre å studere Cell Svar å ekstracellulære Matrix proteiner
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1Faculty of Biology, Medicine and Health. Division of Cell Matrix, Biology and Regenerative Medicine, Wellcome Trust Centre for Cell-Matrix Research,The University of Manchester, 2School of Materials,The University of Manchester, 3Blond McIndoe Laboratories, School of Biological Sciences, Faculty of Biology, Medicine and Health, Division of Cell Matrix Biology and Regenerative Medicine, The University of Manchester,Manchester Academic Health Science Centre, 4Department of Plastic Surgery & Burns, Wythenshawe Hospital, Manchester University NHS Foundation Trust,Manchester Academic Health Science Centre, 5Department of Pathology,UMC Utrecht
Chapters
- 00:04Title
- 00:57Design of Pattern Templates and Plasma Cleaning
- 01:52Passivation with PLL-PEG
- 02:54System Calibration
- 04:36Software Set-up and Photopatterning
- 05:29Protein Incubation and Blocking Unspecific Binding Sites
- 06:43Second Round of Photopatterning and Protein Incubation
- 07:39Results: Micropatterning for the Study of Cell Responses to Extracellular Matrix Proteins
- 09:03Conclusion
Vårt overordnede mål er å forstå hvordan cellene forstand ekstracellulære signaler som fører til rettet axonal vekst. Her beskriver vi metodikken for lys-indusert molekylær absorpsjon av proteiner, som brukes til å produsere definerte mikro-mønstre av ekstracellulære matrise komponenter for å studere bestemte hendelser som styrer axon utvekst og pathfinding.
