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通过红外纳米光谱和原子力显微镜对单个蛋白质聚合进行特征化
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
DOI:

12:58 min

September 12, 2019

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Chapters

  • 00:04Title
  • 01:09Atomic Force Microscopy (AFM) Protein Aggregate Morphology Imaging
  • 05:09Infrared (IR) Nanospectroscopy Protein Aggregate Measurement
  • 11:57Results: Individual Protein Aggregated Characterization by IR Nanospectroscopy and AFM
  • 13:30Conclusion

Summary

Automatic Translation

我们描述了红外纳米光谱学和高分辨率原子力显微镜的应用,将蛋白质自组装过程可视化为寡聚体和淀粉样纤维蛋白,这与发病和发展密切相关广泛的人类神经退行性疾病。

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