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Chapters
- 00:04Title
- 00:38Induced Pluripotent Stem Cell (iPSC) Passaging and Maintenance
- 01:40Human Embryonic Stem Cell (hESC)-Qualified Matrix-Coated Plates and Coverslips
- 02:14Selected iPSC-Cardiomyocyte (CM) Plating
- 03:39Intracellular Calcium (Ca2+) Transient Assessment
- 05:42Results: Representative iPSC-CM Phenotypic Characterization, Differentiation Efficiency, and Calcium Amplitude Analyses
- 07:16Conclusion
Her beskriver og validerer vi en metode til konsekvent at generere robuste menneskeskabte pluripotente stamcelle afledte kardiomyocytter og karakterisere deres funktion. Disse teknikker kan hjælpe med at udvikle mekanistisk indsigt i signalering veje, give en platform for storstilet Drug screening, og pålideligt model hjertesygdomme.
