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单细胞微吸气作为荧光激活细胞分类的替代策略,用于巨型病毒混合物分离
JoVE Journal
Biology
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JoVE Journal Biology
Single Cell Micro-aspiration as an Alternative Strategy to Fluorescence-activated Cell Sorting for Giant Virus Mixture Separation
DOI:

09:50 min

October 27, 2019

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Chapters

  • 00:04Title
  • 01:37Amoeba Culture
  • 02:44Propagation of Stock Virus in Amoebae
  • 03:33End-point Dilution and Single Cell Micro-aspiration
  • 06:09PCR Screening
  • 07:06Results: Optimization of the Isolation of Novel Viruses
  • 08:17Conclusion

Summary

Automatic Translation

在这里,我们描述了一个单细胞微吸入方法分离受感染的阿米巴。为了分离受福斯托病毒和未知巨病毒感染的Vermamoeba病毒的病毒亚群,我们开发了下面详述的规程,并展示了其分离两种低丰度的新型巨病毒的能力。

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