Journal
/
Biology
/
Enkelt celle mikro-aspiration som en alternativ strategi for fluorescens-aktiverede celle sortering for Giant virus blanding adskillelse
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Single Cell Micro-aspiration as an Alternative Strategy to Fluorescence-activated Cell Sorting for Giant Virus Mixture Separation

Enkelt celle mikro-aspiration som en alternativ strategi for fluorescens-aktiverede celle sortering for Giant virus blanding adskillelse

DOI:
10.3791/60148-v

•

09:50 min

•

October 27, 2019

•

, , , , ,
1Institut Hospitalo-Universitaire (IHU) – Méditerranée Infection, 2Microbes, Evolution, Phylogeny and Infection (MEΦI), Aix-Marseille Université UM63, Institut de Recherche pour le Développement IRD 198,Assistance Publique – Hôpitaux de Marseille (AP-HM)

Chapters

  • 00:04Title
  • 01:37Amoeba Culture
  • 02:44Propagation of Stock Virus in Amoebae
  • 03:33End-point Dilution and Single Cell Micro-aspiration
  • 06:09PCR Screening
  • 07:06Results: Optimization of the Isolation of Novel Viruses
  • 08:17Conclusion

Summary

Automatic Translation

Automatic Translation

Her beskriver vi en enkelt celle mikro-aspiration metode til adskillelse af inficerede amoebae. For at adskille virale subpopulationer i Vermamoeba vermiformis inficeret af Faustovira og ukendte gigantiske vira, vi udviklede protokollen nedenfor og viste sin evne til at adskille to lav-overflod roman Giant vira.

Tags

Single Cell Micro-aspirationFluorescence-activated Cell SortingGiant VirusVirus SeparationVirus IsolationTurnover VirusClandisinal VirusUzibati VirusFaustovirusIndirect StrategyInfected HostMolecular BiologyElectronic MicroscopyVermamoeba VermiformisPYG MediumStarvation MediumMultiplicity Of Infection

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Fluorescence Activated Cell Sorting of Plant Protoplasts

Bromodeoxyuridine (BrdU) Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton

Agarosegelelektroforese til adskillelse af DNA fragmenter

Cell specifik analyse af<em> Arabidopsis</em> Blade Med fluorescensaktiveret cellesortering

Optimeret farvning og spredning modelleringsmetoder for celledeling Overvågning ved hjælp cellesporing Farvestoffer

Isolering af normale og cancer-associeret fibroblaster fra friske væv ved fluorescens-aktiveret cellesortering (FACS)

En strategi for følsomme, Large Scale Kvantitative Metabolomics

En alternativ metode til Sample Preparation med lav Cell Antal for TEM Analyse

Glomerular udvækst som en Ex Vivo Assay at analysere veje involveret i Parietal Epitelial Cell Aktivering

Fluorescensaktiveret cellesortering til isoleractiniske cellepopulationer

Read Article