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Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch’s Membrane
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch’s Membrane

Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch’s Membrane

DOI:
10.3791/57084-v

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08:32 min

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April 12, 2018

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, , , ,
1Department of Ophthalmology and Visual Science,Yale School of Medicine, 2Edward S. Harkness Eye Institute,Columbia University School of Medicine

Chapters

  • 00:05Title
  • 01:04Harvesting of Human Bruch’s Membrane Explants
  • 04:53Culturing of Retinal Pigment Epithelial (RPE) Cells on Human Bruch’s Membrane Explants
  • 06:36Results: Culturing RPE Cells on Aged Bruch’s Membranes
  • 07:19Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to demonstrate the culturing of retinal pigment epithelial (RPE) cells on aged and/or diseased human Bruch's membrane. This method is suitable to study RPE cell behavior on a compromised extracellular matrix.

Tags

Retinal Pigment Epithelial CellsBruch’s MembraneEx Vivo ModelAged HumanAMD EtiologyProteomicsRPE Cell CultureScleraVitreousRetinaOptic NerveAmmonium HydroxideDPBSPTFE Membrane

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