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Journal
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Immunology and Infection
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在胶原蛋白诱发关节炎模型中,用于处理小鼠爪的冷冻粉碎协议,用于评估组织炎症的分子通路
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Immunology and Infection
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JoVE Journal
Immunology and Infection
A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model
Please note that all translations are automatically generated.
Click here for the English version.
在胶原蛋白诱发关节炎模型中,用于处理小鼠爪的冷冻粉碎协议,用于评估组织炎症的分子通路
DOI:
10.3791/60229-v
•
11:03 min
•
October 30, 2019
•
Brian Jones
,
Shannon Hitchcock
,
Matthew Loza
,
Ravi Malaviya
,
Tatiana Ort
,
Fang Shen
1
Immunology
,
Janssen Research & Development, Inc.
Chapters
00:04
Title
01:55
Cryogenic Freezer Mill-based Pulverization Method
04:11
RNA Extraction
06:36
Protein Extraction
07:57
Results: Evaluating the Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model
09:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
开发了一种使用液氮冷冻机加工鼠爪的低温粉碎方法,以提高从组织中提取的RNA或蛋白质的产量和质量,并分析与炎症相关的分子图谱反应。
Tags
Cryo-pulverization
Collagen-induced Arthritis Model
Molecular Profiling
Proteomic Analysis
Transcriptional Profiling
RNA Isolation
Arthritis Research
Preclinical Model
Freezer Mill
Liquid Nitrogen
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