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Journal
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Developmental Biology
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果蝇黑色素
血脑屏障完整性分析
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Assay for Blood-brain Barrier Integrity in
Drosophila melanogaster
Please note that all translations are automatically generated.
Click here for the English version.
果蝇黑色素
血脑屏障完整性分析
DOI:
10.3791/60233-v
•
09:08 min
•
September 18, 2019
•
Matthew J. Davis
,
Danielle Talbot
,
Jennifer Jemc
1
Department of Biology
,
Loyola University Chicago
Chapters
00:04
Title
00:40
Embryo Collection
03:21
Larval Collection
04:05
Needle Preparation and Specimen Injection
05:43
Embryo Imaging
06:17
Larval Dissection and Imaging
07:27
Results: Representative Blood-Brain Barrier (BBB) Formation Analysis
08:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
血脑屏障完整性对神经系统功能至关重要。在
果蝇黑色素仪
中,血脑屏障是由胚胎发育晚期的胶质细胞形成的。该协议描述了
在D.melanogaster
胚胎和第三星幼虫中进行血脑屏障形成和维护的测定方法。
Tags
Blood-brain Barrier
Drosophila Melanogaster
Embryo Collection
Barrier Permeability
Apple Juice Agar
PbTx
Dechorionation
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