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球状外生长作为前维沃分析分析涉及的路径的表皮上皮细胞激活
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Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation
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球状外生长作为前维沃分析分析涉及的路径的表皮上皮细胞激活

DOI:
10.3791/60324-v

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06:39 min

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August 19, 2020

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, , , , , ,
1Radboud Institute for Molecular Life Sciences, Department of Pathology,Radboud University Medical Center, 2Radboud Institute for Molecular Life Sciences, Amalia Children’s Hospital, Department of Paediatric Nephrology,Radboud University Medical Center, 3Radboud Institute for Health Sciences, Department of Nephrology,Radboud University Medical Center, 4Radboud Institute for Molecular Life Sciences, Department of Nephrology,Radboud University Medical Center

Chapters

  • 00:04Introduction
  • 00:39Mouse Kidney Dissection
  • 01:04Isolation of Glomeruli from Mouse Kidney
  • 02:25Culturing of Glomerular Outgrowth
  • 03:40Analysis of Parietal Epithelial Cell Proliferation
  • 04:47Results: Analysis of Glomerular Outgrowth
  • 06:08Conclusion

Summary

Automatic Translation

Automatic Translation

本文介绍了一种培养和分析从小鼠肾脏分离出的封装球状上皮细胞生长的方法。此方法可用于研究涉及单骨上皮细胞增殖和迁移的途径。

Tags

Glomerular OutgrowthParietal Epithelial Cell ActivationKidneyEx Vivo AssayCellular ProcessesPrimary CellsGlomeruliKidney IsolationCell CultureMicroscopy

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