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光遺伝学的変調型心筋細胞活性の電気機械評価

JoVE Journal
Medicine

This content is Free Access.

JoVE Journal Medicine

Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity

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光遺伝学的変調型心筋細胞活性の電気機械評価

7,726 Views

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12:52 min

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March 05, 2020

DOI:

10.3791/60490-v

DOI:

10.3791/60490-v

•

12:52 min

March 05, 2020

•

4 Views

Ramona A. Kopton^1,2,3, Cinthia Buchmann^1,2, Robin Moss^1,2, Peter Kohl^1,2, Rémi Peyronnet^1,2, Franziska Schneider-Warme^1,2

¹Institute for Experimental Cardiovascular Medicine, University Heart Center Freiburg-Bad Krozingen,Medical Center-University of Freiburg, ²Faculty of Medicine,University of Freiburg, ³Faculty of Biology,University of Freiburg

Summary

ウサギ心筋細胞におけるGtACR1活性化の電気機械効果を評価するプロトコルを提示する。細胞分離、培養、アデノウイルス伝達、パッチクランプ、炭素繊維技術による機能実験に関する詳細な情報を提供します。

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