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光遗传学调节心细胞活性的机电评估
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity
Please note that all translations are automatically generated.
Click here for the English version.
光遗传学调节心细胞活性的机电评估
DOI:
10.3791/60490-v
•
12:52 min
•
March 05, 2020
•
Ramona A. Kopton
2,3
,
Cinthia Buchmann
2
,
Robin Moss
2
,
Peter Kohl
2
,
Rémi Peyronnet
2
,
Franziska Schneider-Warme
2
1
Institute for Experimental Cardiovascular Medicine, University Heart Center Freiburg-Bad Krozingen
,
Medical Center-University of Freiburg
,
2
Faculty of Medicine
,
University of Freiburg
,
3
Faculty of Biology
,
University of Freiburg
Chapters
00:05
Introduction
01:33
Cell Isolation
02:57
Cardiomyocyte Culture
04:17
Patch-Clamp Experiment
05:44
Carbon Fiber Preparation
07:40
Cardiomyocyte Contraction Force Recording
10:23
Results: Representative Patch-Clamp and Carbon Fiber Recordings
11:44
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了一个评估GtACR1激活在兔子心肌细胞中的机电效应的协议。我们提供细胞分离、培养和抗病毒转导的详细信息,以及贴片夹和碳纤维技术的功能实验。
Tags
Optogenetics
Cardiomyocyte
Electromechanical Assessment
GtACR1
Patch Clamp
Sarcomere Tracking
Carbon Fiber
Optical Defibrillation
Ventricular Cardiomyocytes
Smooth Muscle
Single-cell Muscle
Cell Stretching
Cell Dissociation
Cell Culture
Laminin
Article
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