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光遗传学调节心细胞活性的机电评估
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity
DOI:

12:52 min

March 05, 2020

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Chapters

  • 00:05Introduction
  • 01:33Cell Isolation
  • 02:57Cardiomyocyte Culture
  • 04:17Patch-Clamp Experiment
  • 05:44Carbon Fiber Preparation
  • 07:40Cardiomyocyte Contraction Force Recording
  • 10:23Results: Representative Patch-Clamp and Carbon Fiber Recordings
  • 11:44Conclusion

Summary

Automatic Translation

我们提出了一个评估GtACR1激活在兔子心肌细胞中的机电效应的协议。我们提供细胞分离、培养和抗病毒转导的详细信息,以及贴片夹和碳纤维技术的功能实验。

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