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Journal / Medicine / Elektromekanisk vurdering af optogenetisk…
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Medicine

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Elektromekanisk vurdering af optogenetisk moduleret kardiomyocyt aktivitet
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Elektromekanisk vurdering af optogenetisk moduleret kardiomyocyt aktivitet

Article DOI: 10.3791/60490-v • 12:52 min • March 5th, 2020
March 5th, 2020 •
1,2,3, 1,2, 1,2, 1,2, 1,2, 1,2
1Institute for Experimental Cardiovascular Medicine, University Heart Center Freiburg-Bad Krozingen, Medical Center-University of Freiburg, 2Faculty of Medicine, University of Freiburg, 3Faculty of Biology, University of Freiburg

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Vi præsenterer en protokol til evaluering af de elektromekaniske virkninger af GtACR1 aktivering i kanin kardiomyocytter. Vi giver detaljerede oplysninger om celleisolation, dyrkning og adenoviral transduktion og om funktionelle eksperimenter med patch-clamp og kulfiberteknikker.

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Medicin Naturlig anion channelrhodopsin GtACR1 Guillardia theta hjerte kardiomyocytter optogenetik handling potentiale hjerte elektrofysiologi kulfiber teknik kontraktilitet kraft måling mekanik
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