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小鼠体内合成蛋白的定量测量
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Neuroscience
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JoVE Journal Neuroscience
Quantitative Measurement of Intrathecally Synthesized Proteins in Mice
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小鼠体内合成蛋白的定量测量

DOI:
10.3791/60495-v

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08:23 min

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November 29, 2019

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, , , , ,
1Department of Neurology,Geisel School of Medicine & Dartmouth-Hitchcock Medical Center, 2Program in Experimental and Molecular Medicine,Dartmouth College

Chapters

  • 00:04Title
  • 01:18Serum Collection: Survival Procedure
  • 02:44CerebroSpinal Fluid (CSF) Collection: Survival Procedure
  • 05:05Serum and CSF Collection: Non-Survival Procedure
  • 06:07Protein Analysis
  • 07:02Results: Representative Blood-Brain Barrier Function and Intrathecal IgG Synthesis in Relapsing Experimental Autoimmune Encephalomyelitis (R-EAE) and Theiler’s Murine Encephalomyelitis Virus-Induced Demyelinating Disease (TMEV-IDD)
  • 07:53Conclusion

Summary

Automatic Translation

Automatic Translation

脊髓液蛋白水平升高可能是血浆蛋白在改变的血脑屏障上扩散的结果，也可以是血脑内合成的结果。本文提出了一种优化的测试方案，有助于区分这两种情况，并提供对内代谢蛋白的定量测量。

Tags

Quantitative MeasurementIntrathecally Synthesized ProteinsMouse ModelsNeurological DisordersCSF Protein LevelsBlood-CSF Barrier IntegrityCentral Nervous System DiseasesRetro-orbital BleedingSerum CollectionCerebral Spinal Fluid Collection

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