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Journal
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Immunology and Infection
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使用IBA1和TMEM119进行小鼠脑微胶质密度、形态学和周围骨髓细胞渗透分析的免疫荧光染色
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain
Please note that all translations are automatically generated.
Click here for the English version.
使用IBA1和TMEM119进行小鼠脑微胶质密度、形态学和周围骨髓细胞渗透分析的免疫荧光染色
DOI:
10.3791/60510-v
•
10:40 min
•
October 27, 2019
•
Fernando González Ibanez
2
,
Katherine Picard
2
,
Maude Bordeleau
3
,
Kaushik Sharma
2,4
,
Kanchan Bisht
2,4
,
Marie-Ève Tremblay
2
1
Axe Neurosciences
,
Centre de Recherche du CHU de Québec-Université Laval
,
2
Département de Médecine Moléculaire, Faculté de Médecine
,
Université Laval
,
3
Integrated Program in Neuroscience
,
McGill University
,
4
Center for Brain Immunology and Glia (BIG)
,
University of Virginia
Chapters
00:04
Title
01:00
Density and Distribution Analysis
05:50
Morphology Analysis
09:01
Results: Immunofluorescence Staining Using IBA1 and TMEM119
10:06
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了IBA1和TMEM119免疫荧光结化的分步工作流程,除了分析微胶质密度、分布和形态,以及小鼠脑组织中外周髓细胞渗透。
Tags
Immunofluorescence
IBA1
TMEM119
Microglia
Peripheral Myeloid Cell
Density
Morphology
Infiltration
Mouse Brain
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