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Journal
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Immunology and Infection
/
将葛兰阴性细菌的细胞包络分离到内膜和外膜分数,对
阿辛托巴基的
培养素进行技术调整
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for
Acinetobacter baumannii
Please note that all translations are automatically generated.
Click here for the English version.
将葛兰阴性细菌的细胞包络分离到内膜和外膜分数,对
阿辛托巴基的
培养素进行技术调整
DOI:
10.3791/60517-v
•
10:24 min
•
April 10, 2020
•
Melina B. Cian*
1
,
Nicole P. Giordano*
1
,
Joshua A. Mettlach*
1
,
Keaton E. Minor*
1
,
Zachary D. Dalebroux
1
Department of Microbiology and Immunology
,
University of Oklahoma Health Sciences Center
Chapters
00:04
Introduction
00:55
Preparation of Bacteria for Membrane Extraction
02:18
Dissociation of the Outer Membrane and Plasmolysis
03:56
Pressurized Homogenization and Lysis
04:35
Total Membrane Fractionation
05:34
Separating the Dual Membranes
08:00
Results: Purity and Quality of Inner and Outer Membrane Fractions
09:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
革兰氏阴性细菌产生两个空间隔离膜。外膜由围皮层和气膜层从内膜分割。分离这些微生物的双层的能力对于了解它们的生理和发病机制至关重要。
Tags
Cell Envelope
Gram-negative Bacteria
Inner Membrane
Outer Membrane
Lipids
Proteins
Sugars
Antibiotic Killing
Antimicrobial Resistance
Disease Pathogenesis
Acinetobacter Baumannii
Cell Fractionation
Detergent-free
Semi-native Environment
Optical Density
Centrifugation
Lysozyme
EDTA
Protease Inhibitor
Magnesium Chloride
Nuclease
Article
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