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Bioengineering
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中国仓鼠卵巢细胞培养中高通量自动化微生物反应器工艺优化
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation
Please note that all translations are automatically generated.
Click here for the English version.
中国仓鼠卵巢细胞培养中高通量自动化微生物反应器工艺优化
DOI:
10.3791/60577-v
•
09:28 min
•
May 18, 2020
•
Tamanna Nagraik
,
Alina Gonzalez Salcedo
,
Dörte Solle
,
Thomas Scheper
1
Institute for Technical Chemistry
,
Gottfried-Wilhelm-Leibniz Universität Hannover
Chapters
00:04
Introduction
00:43
Writing the Recipe in the Automated Micro-Bioreactor Software
04:25
Design of Experiment in the Automated Micro Bioreactor
06:22
Execution of the Experiment in the Work Packets and Analysis of Experimental Results
07:25
Results: DOE Experiment and Response Contour Plots
08:44
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了一个详细的过程,在自动化的微生物反应器中运行实验设计,然后使用蛋白质A柱进行细胞收获和蛋白质定量。
Tags
Process Optimization
High Throughput
Automated Micro-bioreactors
Chinese Hamster Ovary (CHO) Cell Cultivation
Design Of Experiments (DOE)
Data Analysis
Pre-culture Procedure
Cultivation Conditions
Media Addition
Inoculum Addition
Feed Addition
Glucose Addition
Anti-foam Addition
Software Interface
Automated Liquid Handling
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