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一种从小鼠中分离大脑围取体的高输出方法
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
A High Output Method to Isolate Cerebral Pericytes from Mouse
Please note that all translations are automatically generated.
Click here for the English version.
一种从小鼠中分离大脑围取体的高输出方法
DOI:
10.3791/60588-v
•
06:49 min
•
January 14, 2020
•
Anupriya Mehra
,
Lucie Dehouck
,
Elodie Vandenhaute
,
Marc Fatar
,
Laurence Fenart
,
Fabien Gosselet
1
Laboratory of the Blood Brain Barrier
,
University of Artois
,
2
Department of Neurology, Universitätsmedizin Mannheim
,
Heidelberg University
Chapters
00:04
Title
00:44
Brain Tissue Recovery and Meninges Removal
01:31
Homogenization
02:18
Vascular Fraction Isolation
04:32
Cerebral Pericyte Proliferation
05:25
Results: Representative Cell Morphology, Marker Expression, and Culture Purity
06:25
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了提取鼠脑围细胞的规程。该方案基于无抗生素富集定向围取剂,是体外研究提供高纯度、高产量的宝贵工具,从而减少了实验动物的数量。
Tags
Cerebral Pericytes
Pericyte Isolation
Mouse Brain
In Vitro Studies
Cell Culture
Cell Yield
High Purity
Homogenization
Vascular Fraction
Bovine Serum Albumin Dextran
Cell Suspension
Filtration
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