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Biochemistry
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通过基因表达分析的改良方法从谷物种子获取高质量的转录数据
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
Please note that all translations are automatically generated.
Click here for the English version.
通过基因表达分析的改良方法从谷物种子获取高质量的转录数据
DOI:
10.3791/60602-v
•
07:18 min
•
May 21, 2020
•
Vito M. Butardo Jr.
,
Christiane Seiler
,
Nese Sreenivasulu
,
Markus Kuhlmann
1
Department of Chemistry and Biotechnology
,
Swinburne University of Technology
,
2
Department of Molecular Genetics, Heterosis Research Group
,
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
,
3
Grain Quality and Nutrition Center, Applied Functional Genomics Cluster
,
International Rice Research Institute
Chapters
00:04
Introduction
00:40
Microarray Hybridization
03:09
Microarray Scanning
05:05
Results: Representative Transcriptome Data Analyses
06:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
提出了谷物转录分析的方法。基于微阵列的基因表达分析从谷物中分离高质量的总RNA开始,并随着cDNA的生成而继续。在cRNA标签和微阵列杂交后,提出了信号检测和质量控制的建议。
Tags
Microarray Hybridization
Gene Expression Profiling
Cereal Seeds
Transcriptome Data
High-throughput Sequencing
Blocking Agent
Fragmentation Mix
Hybridization Buffer
Microarray Slide
Hybridization Assembly
Hybridization Oven
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