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Ex Vivo Pressurized Hippocampal Capillary-Parenchymal Arteriole Preparation for Functional Study
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JoVE Journal Neuroscience
Ex Vivo Pressurized Hippocampal Capillary-Parenchymal Arteriole Preparation for Functional Study

Ex Vivo Pressurized Hippocampal Capillary-Parenchymal Arteriole Preparation for Functional Study

DOI:
10.3791/60676-v

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09:15 min

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December 18, 2019

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, ,
1Department of Anesthesiology,University of Colorado Anschutz Medical Campus, 2Department of Neurological Sciences,University of Vermont Larner College of Medicine, 3Department of Pharmacology,University of Colorado Anschutz Medical Campus

Chapters

  • 00:04Title
  • 00:32Hippocampus Dissection and Isolation
  • 02:22Hippocampal Arteriole Isolation
  • 03:10Hippocampal Capillary-Parenchymal Arteriole Cannulation
  • 04:27Pressure Myography
  • 05:55Capillary End Focal Stimulation
  • 07:27Results: Representative Effects of Focal Stimulation on Upstream Arteriolar Diameter Dilation
  • 08:42Conclusion

Summary

Automatic Translation

Automatic Translation

The present manuscript details how to isolate hippocampal arterioles and capillaries from the mouse brain and how to pressurize them for pressure myography, immunofluorescence, biochemistry, and molecular studies.

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Keywords: Ex VivoHippocampal Capillary-parenchymal ArterioleFunctional StudyDissectionMouse BrainHippocampusArteriole IsolationTransverse ArteryMOPS Buffered SalineCerebellumThalamusCorpus CallosumCerebrum

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