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Ex vivo onder druk Hippocampal capillaire-parenchymale Arteriole voorbereiding voor functionele studie
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Ex Vivo Pressurized Hippocampal Capillary-Parenchymal Arteriole Preparation for Functional Study
DOI:

09:15 min

December 18, 2019

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Chapters

  • 00:04Title
  • 00:32Hippocampus Dissection and Isolation
  • 02:22Hippocampal Arteriole Isolation
  • 03:10Hippocampal Capillary-Parenchymal Arteriole Cannulation
  • 04:27Pressure Myography
  • 05:55Capillary End Focal Stimulation
  • 07:27Results: Representative Effects of Focal Stimulation on Upstream Arteriolar Diameter Dilation
  • 08:42Conclusion

Summary

Automatic Translation

Het huidige manuscript laat zien hoe hippocampal arteriolen en haarvaten van de muis hersenen isoleren en hoe ze onder druk kunnen worden gezet voor myografie, immunofluorescentie, biochemie en moleculaire studies.

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