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Ex vivo trykbærende Hippocampal kapillar-Parenchymal arteriole forberedelse til funktionel undersøgelse
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Ex Vivo Pressurized Hippocampal Capillary-Parenchymal Arteriole Preparation for Functional Study
DOI:

09:15 min

December 18, 2019

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Chapters

  • 00:04Title
  • 00:32Hippocampus Dissection and Isolation
  • 02:22Hippocampal Arteriole Isolation
  • 03:10Hippocampal Capillary-Parenchymal Arteriole Cannulation
  • 04:27Pressure Myography
  • 05:55Capillary End Focal Stimulation
  • 07:27Results: Representative Effects of Focal Stimulation on Upstream Arteriolar Diameter Dilation
  • 08:42Conclusion

Summary

Automatic Translation

Det nuværende manuskript beskriver, hvordan man isolerer hippocampus arterioler og kapillærer fra muse hjernen, og hvordan man presser dem til trykmyografi, immunofluorescens, biokemi og molekylære studier.

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