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1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen Second People’s Hospital,First Affiliated Hospital of Shenzhen University, Health Science Center, 2Institute for Cellular Therapeutics,Allegheny-Singer Research Institute, 3School of Pharmaceutical Sciences,Wenzhou Medical University, 4Xenotransplantation Program/Department of Surgery,University of Alabama at Birmingham, 5Jiangsu Key Laboratory of Xenotransplantation,Nanjing Medical University
Chapters
- 00:04Title
- 01:13Islet Preparation
- 05:39Inguinal Subcutaneous White Adipose Tissue (ISWAT) Islet Transplantation
- 08:13Results: Representative Islet Morphology and Function
- 09:24Conclusion
In questo protocollo, viene descritto un metodo di isolamento murine ilet e trapianto nel tessuto adiposo bianco sottocutaneo inguinale. Le isole di murine singeniche isolate vengono trapiantate in un recipiente murino utilizzando un idrogel di membrana sotterranea. Viene monitorato il livello di glucosio nel sangue dei destinatari e viene eseguita l'analisi istologia degli innesti di islet.
