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1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen Second People’s Hospital,First Affiliated Hospital of Shenzhen University, Health Science Center, 2Institute for Cellular Therapeutics,Allegheny-Singer Research Institute, 3School of Pharmaceutical Sciences,Wenzhou Medical University, 4Xenotransplantation Program/Department of Surgery,University of Alabama at Birmingham, 5Jiangsu Key Laboratory of Xenotransplantation,Nanjing Medical University
Chapters
- 00:04Title
- 01:13Islet Preparation
- 05:39Inguinal Subcutaneous White Adipose Tissue (ISWAT) Islet Transplantation
- 08:13Results: Representative Islet Morphology and Function
- 09:24Conclusion
Neste protocolo, um método de isolamento e transplante de isto de murina no tecido adiposo branco subcutâneo inguinal é descrito. Ishotas singeneicas isoladas são transplantadas em um receptor de murina usando um hidrogel de membrana do porão. O nível de glicemia dos receptores é monitorado, e a análise de histologia dos enxertos de lumiçal é realizada.
