/
/
Analyse av ikke-menneskelige primater bukspyttkjertel oksygenforbruk
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Molecular Physiology and Biophysics,Vanderbilt University, 2Agilent Technologies, 3Department of Biological Sciences,Vanderbilt University, 4Department of Veterans Affairs Tennessee Valley, 5Department of Medicine,Vanderbilt University Medical Center, 6Department of Cell and Developmental Biology,Vanderbilt University
Chapters
- 00:05Title
- 01:12Spheroid Microplate Preparation
- 02:03Sensor Cartridge Hydration
- 02:36Sensor Cartridge Preparation
- 03:00Pancreatic Islet Transfer
- 04:19Oxygen Consumption Assay
- 05:06Results: Representative Pancreatic Islet Oxygen Consumption Analyses
- 06:09Conclusion
Denne protokoll demonstrerer nøyaktig og reproduserbar måling av oksygen forbruket i ikke-menneskelige primater bukspyttkjertelen holmer. Holmen lasting teknikker og belegg av mikroplate gir et rammeverk for effektiv måling av åndedrett i andre typer kultivert spheroids.
