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Journal
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Cancer Research
/
靶性抗原特异性治疗抗体的肿瘤及组织分布分析
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody
Please note that all translations are automatically generated.
Click here for the English version.
靶性抗原特异性治疗抗体的肿瘤及组织分布分析
DOI:
10.3791/60727-v
•
07:36 min
•
May 16, 2020
•
Gururaj Shivange*
1,2
,
Tanmoy Mondal*
1,2
,
Evan Lyerly
2
,
Jeremy Gatesman
,
Jogender Tushir-Singh
2
1
Laboratory of Novel Biologics
,
University of Virginia School of Medicine
,
2
Department of Biochemistry and Molecular Genetics, UVA Cancer Center
,
University of Virginia School of Medicine
,
3
Center for Comparative Medicine
,
University of Virginia
Chapters
00:04
Introduction
01:02
Expression and Purification of Antibodies
03:22
Fluorescent Labeling
04:10
Antibody Localization Using an In Vivo Imaging System
06:05
Results: Live Imaging of Human OVCAR3 Tumor Bearing Mice
06:56
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了一个方案,研究小鼠肿瘤异种移植模型中抗体的体内定位。
Tags
Antibody Distribution
Tumor Localization
Antibody Screening
CHO Cell Expression
Antibody Purification
Protein A Column
Real-time Monitoring
SPECT
PET
Cost-effective Technique
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