Journal
/
Cancer Research
/
靶性抗原特异性治疗抗体的肿瘤及组织分布分析
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody
Please note that all translations are automatically generated. Click here for the English version.

靶性抗原特异性治疗抗体的肿瘤及组织分布分析

DOI:
10.3791/60727-v

•

07:36 min

•

May 16, 2020

•

, , , ,
1Laboratory of Novel Biologics,University of Virginia School of Medicine, 2Department of Biochemistry and Molecular Genetics, UVA Cancer Center,University of Virginia School of Medicine, 3Center for Comparative Medicine,University of Virginia

Chapters

  • 00:04Introduction
  • 01:02Expression and Purification of Antibodies
  • 03:22Fluorescent Labeling
  • 04:10Antibody Localization Using an In Vivo Imaging System
  • 06:05Results: Live Imaging of Human OVCAR3 Tumor Bearing Mice
  • 06:56Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们提出了一个方案，研究小鼠肿瘤异种移植模型中抗体的体内定位。

Tags

Antibody DistributionTumor LocalizationAntibody ScreeningCHO Cell ExpressionAntibody PurificationProtein A ColumnReal-time MonitoringSPECTPETCost-effective Technique

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

单细胞下游特征的靶细胞预富集与全基因组扩增

数字聚合酶链反应法测定零星家族性腺瘤性息肉病患者的基因变异

生成毛因诱导多能干细胞衍生的肿瘤抗原特异性胸腺移民的三维胸腺培养系统

体外肿瘤细胞恢复对 chimeric 抗原受体 t 细胞抗肿瘤功能的预测评价

在Vivo免疫荧光定位中用于评估癌症研究中的治疗和诊断抗体生物分布

利用人类诱导多能干细胞生成肿瘤抗原特异性T细胞

分析肿瘤基因表达因子与CorExplorer门户网站

测量组织肿瘤组织切片的实时药物反应

一 种研究 并靶向乳腺癌脑转移性肿瘤生长的离体脑切片模型

从正常和肿瘤乳腺组织中生成和成像小鼠和人上皮类器官，无需传代

Read Article