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Journal
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Cancer Research
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標的抗原特異的治療抗体の腫瘍と組織分布の分析
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JoVE Journal
Cancer Research
Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody
Please note that all translations are automatically generated.
Click here for the English version.
標的抗原特異的治療抗体の腫瘍と組織分布の分析
DOI:
10.3791/60727-v
•
07:36 min
•
May 16, 2020
•
Gururaj Shivange*
1,2
,
Tanmoy Mondal*
1,2
,
Evan Lyerly
2
,
Jeremy Gatesman
,
Jogender Tushir-Singh
2
1
Laboratory of Novel Biologics
,
University of Virginia School of Medicine
,
2
Department of Biochemistry and Molecular Genetics, UVA Cancer Center
,
University of Virginia School of Medicine
,
3
Center for Comparative Medicine
,
University of Virginia
Chapters
00:04
Introduction
01:02
Expression and Purification of Antibodies
03:22
Fluorescent Labeling
04:10
Antibody Localization Using an In Vivo Imaging System
06:05
Results: Live Imaging of Human OVCAR3 Tumor Bearing Mice
06:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
ここでは、マウス腫瘍異種移植片モデルにおける抗体のin vivo局在化を研究するプロトコルを提示する。
Tags
Antibody Distribution
Tumor Localization
Antibody Screening
CHO Cell Expression
Antibody Purification
Protein A Column
Real-time Monitoring
SPECT
PET
Cost-effective Technique
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