JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Bioengineering
/
生物材料膜上人与羊角膜内皮细胞层的生长
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Bioengineering
Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes
Please note that all translations are automatically generated.
Click here for the English version.
生物材料膜上人与羊角膜内皮细胞层的生长
DOI:
10.3791/60762-v
•
05:20 min
•
February 06, 2020
•
Jennifer Walshe
,
Najla Al Khaled Abdulsalam
,
Shuko Suzuki
,
Traian V. Chirila
3,4,5,6,7,8
,
Damien G. Harkin
3,4
1
Queensland Eye Institute
,
2
King Faisal University
,
3
School of Biomedical Sciences, Faculty of Health
,
Queensland University of Technology
,
4
Institute of Health and Biomedical Innovation
,
Queensland University of Technology
,
5
Science and Engineering Faculty
,
Queensland University of Technology
,
6
Faculty of Medicine
,
University of Queensland
,
7
Australian Institute for Bioengineering and Nanotechnology
,
University of Queensland
,
8
Faculty of Science
,
University of Western Australia
Chapters
00:05
Introduction
00:35
Explant Dissection and Cell Culture Procedure
01:52
Growth of Corneal Endothelial Cell Layers on Biomaterial Membranes
03:28
Results: Corneal Endothelial Cell Cultures Derived from Sheep and Human Explants
04:47
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该协议描述了从人类或绵羊组织外植植物中建立和生长角膜内皮细胞培养的关键步骤。提出了一种在膜状生物材料上对角膜内皮细胞进行亚培养的方法。
Tags
Corneal Endothelial Cells
Cell Culture
Biomaterial Membranes
Descemet’s Membrane
Micro Boyden Chamber
Epithelial To Mesenchymal Transition
Cell Isolation
Cell Expansion
Cell Subculture
Primary Cells
Attachment Factor
Culture Medium
Cell Suspension
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
片上内皮炎症表型
质量流式细胞术:协议,每日调整和执行细胞的样品上一个CyTOF的质量流式细胞仪
Microstereolithography和静电结合,以产生膜配备有利基的角膜再生
从快速荧光成像在分子扩散法对活细胞的膜在商业显微镜
铁磁金属裸支架对血管内皮细胞捕获和保留
白细胞 - 富血小板纤维蛋白,一种新型生物材料的表征
活细胞层的快速消减带图案微流控探头
胰岛血气 StarPEG Nanocoating 的表面工程
人卵巢组织与工程化内皮细胞联合移植: 一种以细胞为基础的快速灌注与直接分泌的融合策略
巨噬细胞报告器细胞分析,以检查聚合物表面的吸附蛋白层上的收费类似受体介导NF-kB/AP-1信号
Read Article