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形态基因的农业细菌 -顽固玉米近亲繁殖系的介导未成熟胚胎转化
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Agrobacterium
-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
Please note that all translations are automatically generated.
Click here for the English version.
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形态基因的农业细菌 -顽固玉米近亲繁殖系的介导未成熟胚胎转化
DOI:
10.3791/60782-v
•
10:28 min
•
February 14, 2020
•
Alicia Masters
2
,
Minjeong Kang
3,4
,
Morgan McCaw
3
,
Jacob D. Zobrist
3,5
,
William Gordon-Kamm
,
Todd Jones
,
Kan Wang
3
1
Department of Agronomy
,
Iowa State University
,
2
Department of Applied Science and Technology
,
Corteva Agriscience
,
3
Crop Bioengineering Center
,
Iowa State University
,
4
Interdepartmental Plant Biology Major
,
Iowa State University
,
5
Interdepartmental Genetics and Genomics Major
,
Iowa State University
Chapters
00:05
Introduction
01:01
Growing Donor Plants and Harvesting Immature Ears
02:40
Embryo Dissection, Infection, and Co-cultivation
05:54
Selection, Heat Treatment, and Regeneration
07:32
Greenhouse Transplantation and T1 Seed Production
08:08
Results: Representative
Agrobacterium
-mediated Immature Embryo Transformation
09:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
植物形态基因可用于改善顽固基因型的基因转化。这里描述的是三个重要的公共玉米近亲繁殖线的
农业细菌
介导遗传转化(QuickCorn)协议。
Tags
Agrobacterium-mediated
Immature Embryo
Transformation
Recalcitrant
Maize
Inbred Lines
Morphogenic Genes
BABY BOOM
WUSCHEL
QuickCorn
Callus Induction
T-DNA
Cre/loxP
Heat-induced Recombination
Embryogenic Growth
Pollen
Pollination
Immature Embryo Size
Embryo Extraction
Article
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