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Immunology and Infection
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En snabb metod för multispektrala fluorescensavbildning av frysta vävnadssektioner
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Immunology and Infection
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JoVE Journal
Immunology and Infection
A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections
Please note that all translations are automatically generated.
Click here for the English version.
En snabb metod för multispektrala fluorescensavbildning av frysta vävnadssektioner
DOI:
10.3791/60806-v
•
07:50 min
•
March 30, 2020
•
Dinesh Jaishankar
,
Cormac Cosgrove
,
Ryan J. Deaton
,
I. Caroline Le Poole
3
1
Robert Lurie Comprehensive Cancer Center and Department of Dermatology, Feinberg School of Medicine
,
Northwestern University
,
2
Department of Pathology
,
University of Illinois at Chicago
,
3
Department of Microbiology and Immunology, Feinberg School of Medicine
,
Northwestern University
Chapters
00:05
Introduction
00:39
Antibody and Fluorophore Selecting and Staining
01:52
Spectral Library Preparation
02:59
Multispectral Imaging
03:54
Spectral Unmixing
04:37
Analyzing Multispectral Images via Cell Segmentation and Phenotyping
05:52
Results: Representative Multispectral Imaging and Cell Quantification
07:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
Vi beskriver en snabb färgning metod för att utföra multispektrala imaging på frysta vävnader.
Tags
Multispectral Fluorescence Imaging
Biomarker Detection
Co-localization
Rapid Staining Method
Spectral Library
Machine Learning
Image Analysis
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