JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Genetics
/
遺伝子組み換えマウスの高効率生産のための凍結解凍胚の使用
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Genetics
Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
Please note that all translations are automatically generated.
Click here for the English version.
遺伝子組み換えマウスの高効率生産のための凍結解凍胚の使用
DOI:
10.3791/60808-v
•
06:46 min
•
April 02, 2020
•
Hirofumi Nishizono*
1,2,3
,
Mohamed Darwish*
4,5
,
Hideki Uosaki
7
,
Nanami Masuyama
9,10
,
Motoaki Seki
11
,
Hiroyuki Abe
,
Nozomu Yachie
9,10,12,13
,
Ryohei Yasuda
1
Max Planck Florida Institute for Neuroscience
,
2
Life Science Research Center
,
University of Toyama
,
3
Department of Biochemical Engineering, Graduate School of Science and Engineering
,
Yamagata University
,
4
Graduate School of Innovative Life Science
,
University of Toyama
,
5
Department of Biochemistry, Faculty of Pharmacy
,
Cairo University
,
6
Division of Regenerative Medicine, Center for Molecular Medicine
,
Jichi Medical University
,
7
Division of Stem Cell Research and Drug Development, Center for Development of Advanced Medical Technology
,
Jichi Medical University
,
8
Synthetic Biology Division, Research Center for Advanced Science and Technology
,
University of Tokyo
,
9
Institute for Advanced Biosciences
,
Keio University
,
10
Graduate School of Media and Governance
,
Keio University
,
11
Department of Molecular Oncology, Graduate School of Medicine
,
Chiba University
,
12
Department of Biological Sciences, School of Science
,
University of Tokyo
,
13
College of Arts and Sciences
,
University of Tokyo
Chapters
00:05
Introduction
00:35
In Vitro Fertilization
02:23
Embryo Cryopreservation
03:13
Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) Reagent Assembly
04:19
CRISPR Electroporation
05:09
Results: Representative Knockout Mouse Generation Efficiency and Mosaic Rate Evaluation
06:09
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
ここでは、1細胞胚の凍結保存のための改変方法と、遺伝子組換えマウスの効率的な生成のための凍結解凍胚とエレクトロポレーションの使用を組み合わせたプロトコルを提示する。
Tags
Genetically Modified Mice
Freeze-thawed Embryos
In Vitro Fertilization
Super Ovulation
Sperm Capacitation
Cryopreservation
Embryo Electroporation
High-efficiency
Low Mosaic Rate
Protocol
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
再生可能バイオ燃料および生化学的生産のための独創的な酵母の遺伝子工学
遺伝子編集アメリカナマズ、
Ictalurus イシガキダイ
胚に CRISPR/Cas9 蛋白質のマイクロインジェクション
しっかりと段階的な初期の
ショウジョウバエ
の胚からゲノムワイドなクロマチン構造キャプチャ ライブラリの生成
生産およびバキュロ ウイルスの遺伝子治療応用のための浄化
レンチ ウイルス媒介トランスジェニック マウスの生産: シンプルで高効率な創設者の直接の研究法
前立腺癌の遺伝子組み換えマウスモデルからの腫瘍オルガノイドの生成
DNA損傷評価のための彗星アッセイにおける凍結組織の利用
窒素固定熱帯カンナバ科の木
パラスポニア・アンダーソンニ
の変形、ゲノム編集、表現型
細胞分裂のライブイメージングのための
ショウジョウバ
エS2細胞の使用
マグナペアテオリザ
エにおけるクロマチン免疫沈降シーケンシング法を用いたヒストン修飾分布のゲノム解析
Read Article