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Journal
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Genetics
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通过RNA-Seq分析,人类原主角细胞的体外分化特征
JoVE Journal
Genetics
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JoVE Journal
Genetics
Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
Please note that all translations are automatically generated.
Click here for the English version.
通过RNA-Seq分析,人类原主角细胞的体外分化特征
DOI:
10.3791/60905-v
•
07:29 min
•
May 16, 2020
•
Jos G.A. Smits*
1
,
Jieqiong Qu*
1
,
Hanna Niehues
,
Huiqing Zhou
3
1
Department of Molecular Developmental Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences
,
Radboud University, Nijmegen, The Netherlands
,
2
Department of Dermatology, Radboud University Medical Center
,
Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands
,
3
Department of Human Genetics
,
Radboud University Medical Center, Nijmegen, The Netherlands
Chapters
00:04
Introduction
01:10
Human Primary Keratinocyte Differentiation by Contact Inhibition
02:20
RNA-seq Data Analysis
04:53
Results: Keratinocyte Differentiation and Analysis
06:47
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里介绍的是一个逐步的过程,通过接触抑制来分离人类原性角蛋白细胞的体外分化,然后通过RNA-seq分析在分子水平上进行表征。
Tags
In Vitro
Keratinocyte Differentiation
RNA-seq
2D Monolayer
Proliferation Medium
Differentiation Medium
Cell Seeding Density
RNA Isolation
CDNA Conversion
Library Preparation
Next-generation Sequencing
Read Mapping
DESeq2
RLD Normalization
VST Normalization
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