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Mikrofluidic Produktion af Lysolipid-Holdige Temperatur-Følsomme Liposomer
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes
DOI:

09:51 min

March 03, 2020

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Chapters

  • 00:05Introduction
  • 01:06Equipment Setup
  • 02:46Lysolipid-Containing Temperature-Sensitive Liposome (LTSL) Preparation
  • 04:48Remote Doxorubicin (DOX) Loading
  • 05:48Laser Heating and Triggered Release
  • 07:13Results: Representative LTSL10 Characterization, Loading, and Photothermal Heat Triggering
  • 08:59Conclusion

Summary

Automatic Translation

Protokollen præsenterer de optimerede parametre for forberedelse termofølsomme liposomer ved hjælp af forskudt sildeben micromixer microfluidics enhed. Dette giver også mulighed for co-indkapsling af doxorubicin og indocyanin grøn i liposomer og fototermisk-udløst frigivelse af doxorubicin for kontrolleret / udløst narkotika frigivelse.

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