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Time-lapse Imaging af bakterielle sværme og den kollektive stress respons
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JoVE Journal Biology
Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response

Time-lapse Imaging af bakterielle sværme og den kollektive stress respons

DOI:
10.3791/60915-v

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06:26 min

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May 23, 2020

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, ,
1Department of Molecular Biology & Biochemistry,University of California, Irvine, 2Department of Physics & Astronomy,University of California, Irvine, 3Department of Veterinary and Animal Sciences,University of Copenhagen

Chapters

  • 00:04Introduction
  • 00:36Swarming Agar Plate Preparation
  • 01:32P. aeruginosa Growth and Plating
  • 03:30Time-Lapse Image Acquisition and Swarm Repulsion Measurement
  • 04:55Results: Representative Time-lapse Swarming and Collective Stress Response Imaging
  • 05:42Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver en simpel metode til at producere høj opløsning time-lapse film af Pseudomonas aeruginosa sværme, der reagerer på bakteriofage (fag) og antibiotika stress ved hjælp af en flatbed dokument scanner. Denne procedure er en hurtig og enkel metode til overvågning af sværmer dynamik og kan tilpasses til at studere motilitet og vækst af andre bakteriearter.

Tags

Time-lapse ImagingBacterial SwarmingCollective Stress ResponsePseudomonas AeruginosaBacteriophageAntibioticsSwarming Agar PlatesDehumidifierDocument ScannerSwarming DynamicsPhage InfectionAntibiotic TreatmentConcentric Circle Plating

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