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JoVE Journal
Chemistry

This content is Open Access.

Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy
 

Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy

Article doi: 10.3791/60934
March 16th, 2020

Summary March 16th, 2020

Covalent attachment of probe molecules to atomic force microscopy (AFM) cantilever tips is an essential technique for the investigation of their physical properties. This allows us to determine the stretching force, desorption force and length of polymers via AFM-based single molecule force spectroscopy with high reproducibility.

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