JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Chemistry
/
基于AFM的力光谱单分子共价附价
JoVE Journal
Chemistry
This content is Free Access.
JoVE Journal
Chemistry
Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy
Please note that all translations are automatically generated.
Click here for the English version.
基于AFM的力光谱单分子共价附价
DOI:
10.3791/60934-v
•
10:37 min
•
March 16, 2020
•
Adrianna Kolberg
,
Christiane Wenzel
,
Thorsten Hugel
3
,
Markus Gallei
,
Bizan N. Balzer
3
1
Institute of Physical Chemistry
,
Albert-Ludwigs-Universität Freiburg
,
2
Chair in Polymer Chemistry
,
Saarland University
,
3
Cluster of Excellence livMatS at FIT – Freiburg Center for Interactive Materials and Bioinspired Technologies
,
University of Freiburg
Chapters
00:04
Introduction
00:52
Surface Activation, Silanization, and PEGylation
03:03
Covalent Polymer Attachment
04:21
Determining the Inverse Optical Lever Sensitivity (InVOLS)
06:13
Determining the AFM Cantilever Force Constant
07:06
Data Collection
08:10
Results: Single-Molecule Stretching and Desorption
09:44
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
探针分子附于原子力显微镜(AFM)悬臂尖端是研究其物理特性的关键技术。这使我们能够通过基于 AFM 的单分子力光谱,确定聚合物的拉伸力、解吸力和长度,具有高可重复性。
Tags
Single Molecule Force Spectroscopy
AFM-based Force Spectroscopy
Covalent Attachment
Polymer Binding
Silane-PEG-mal
Plasma Activation
Toluene
PEG
Polystyrene
Polynipam
Capillary Forces
Control Samples
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
BMP-2的共价结合利用表面上的自组装单层膜法
Preparation of Silica Nanoparticles Through Microwave-assisted Acid-catalysis
活化分子,离子和固体颗粒与声空化
洞察氨基酸和多肽与无机材料的使用相互作用的单分子力谱
电化学阻抗谱作为电化学速率常数估计的一种工具
信息承载Peptoids及其序列定向动态共价自组装的合成
聚类诱导解吸/电电质谱法对复杂分子及其在表面的反应分析
用于从单一DNA分子大规模平行蛋白质合成的Femtoliter滴体阵列
在水/空气界面上掺杂与疏水有机分子混合脂膜的自组装
原子力显微镜与红外光谱相结合,作为探测单一细菌化学的工具
Read Article