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Neuroscience
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激光捕获胶质瘤分区域的微切,用于肿瘤内异质性、肿瘤流和入侵的空间和分子表征
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion
Please note that all translations are automatically generated.
Click here for the English version.
激光捕获胶质瘤分区域的微切,用于肿瘤内异质性、肿瘤流和入侵的空间和分子表征
DOI:
10.3791/60939-v
•
09:09 min
•
April 12, 2020
•
Andrea Comba
2,4
,
Patrick J. Dunn
2,4
,
Phillip E. Kish
3
,
Padma Kadiyala
2,4
,
Alon Kahana
,
Maria G. Castro
2,4
,
Pedro R. Lowenstein
2,4
1
Dept. of Neurosurgery
,
University of Michigan Medical School
,
2
Dept. of Cell and Developmental Biology
,
University of Michigan Medical School
,
3
Dept. of Ophthalmology & Visual Science
,
University of Michigan Medical School
,
4
Rogel Cancer Center
,
University of Michigan Medical School
Chapters
00:05
Introduction
01:17
Animal Perfusion and Brain Preservation
02:01
Glioma Brain Tumors Tissue Cryopreservation
02:52
Frozen Brain Tumor Tissue Sectioning
03:58
Cryopreserved Brain Tissue Section Fixation and Staining
04:48
Laser Capture Microdissection
06:30
Results: Representative RNA Quality Control and Library Preparation
07:55
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
激光微解剖(LMD)是一种敏感且高度可重复的技术,可用于揭示介导胶质瘤异质性和入侵的途径。在这里,我们描述了一个优化的协议,使用激光LMD从胶质瘤组织中分离离散区域,然后进行转录分析。
Tags
Laser Microdissection
RNA Sequencing
Intratumor Heterogeneity
Mesenchymal Cells
Tumor Invasion
Tumor Morphology
Molecular Profiles
Tumor Progression
Therapeutic Targets
Bioluminescence
Perfusion
Cryopreservation
Spatial Characterization
Molecular Characterization
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