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Journal / Neuroscience / 激光捕获胶质瘤分区域的微切，用于肿瘤内异质性、肿瘤流和入侵的空间和分子表征…
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JoVE Journal Neuroscience
激光捕获胶质瘤分区域的微切，用于肿瘤内异质性、肿瘤流和入侵的空间和分子表征
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激光捕获胶质瘤分区域的微切，用于肿瘤内异质性、肿瘤流和入侵的空间和分子表征

Article DOI: 10.3791/60939-v • 09:09 min • April 12th, 2020
April 12th, 2020 •
1,2,4, 1,2,4, 1,3, 1,2,4, 3, 1,2,4, 1,2,4
1Dept. of Neurosurgery, University of Michigan Medical School, 2Dept. of Cell and Developmental Biology, University of Michigan Medical School, 3Dept. of Ophthalmology & Visual Science, University of Michigan Medical School, 4Rogel Cancer Center, University of Michigan Medical School

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Summary
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激光微解剖（LMD）是一种敏感且高度可重复的技术，可用于揭示介导胶质瘤异质性和入侵的途径。在这里，我们描述了一个优化的协议，使用激光LMD从胶质瘤组织中分离离散区域，然后进行转录分析。

Tags

神经科学， 第158期， 胶质瘤， 激光微切， 转录组分析， RNA 完整性， 肿瘤异质性， 入侵， 分子靶
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