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Developmental Biology
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神经石生长分析与神经毒性评估与人类神经原生细胞衍生神经元
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Developmental Biology
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JoVE Journal
Developmental Biology
A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons
Please note that all translations are automatically generated.
Click here for the English version.
神经石生长分析与神经毒性评估与人类神经原生细胞衍生神经元
DOI:
10.3791/60955-v
•
07:41 min
•
August 06, 2020
•
Amir Bagheri
2
,
Seyedeh Fatemeh Razavipour
,
Claes Wahlestedt
,
Seyed Javad Mowla
,
Mohammad Ali Faghihi
4
1
Department of Molecular Genetics, Faculty of Biological Sciences
,
Tarbiat Modares University
,
2
Center for Therapeutic Innovation and Department of Psychiatry & Behavioral Sciences
,
University of Miami Miller School of Medicine
,
3
Department of Biochemistry and Molecular Biology
,
University of Miami Miller School of Medicine
,
4
Persian BayanGene Research and Training Center
Chapters
00:04
Introduction
00:51
Passaging and Freezing the hNPCs
02:50
Image Acquisition and Analysis
04:25
Neurotoxicity Assessment
06:10
Results: Neurite Outgrowth Assay and Neurotoxicity Assessment with Small Molecule Compounds
07:13
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
提出的方案描述了一种小分子化合物的神经质生长测定和神经毒性评估方法。
Tags
Neurite Outgrowth Assay
Neurotoxicity Assessment
Human Neural Progenitor Cells
Neurons
Induced Pluripotent Stem Cells
Embryonic Stem Cells
Neurosphere
Cell Dissociation
Cell Culture
Cryopreservation
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