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Journal
/
Biology
/
测量
在C.elegan的
RAN肽毒性
JoVE Journal
Biology
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JoVE Journal
Biology
Measuring RAN Peptide Toxicity in
C. elegans
Please note that all translations are automatically generated.
Click here for the English version.
测量
在C.elegan的
RAN肽毒性
DOI:
10.3791/61024-v
•
10:49 min
•
April 30, 2020
•
Paige Rudich
,
Carley Snoznik
,
Noah Puleo
,
Todd Lamitina
2
1
Graduate Program in Cell Biology and Molecular Physiology
,
University of Pittsburgh
,
2
Department of Pediatrics
,
University of Pittsburgh School of Medicine
Chapters
00:04
Introduction
01:05
Measurement of the Developmental Toxicity of RAN Peptides following RNAi-based Gene Knockdown
05:38
Post-developmental RAN Peptide Paralysis Assay
08:02
Results: Toxicity of RAN Peptides
10:04
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
重复相关非ATG相关转化产品是几种重复扩张性疾病的病原特征。所述协议的目的是使用模型系统
C.elegans
的行为和细胞测定来评估这些肽引起的毒性。
Tags
RAN Peptides
Repeat Expansion Neurodegenerative Disorders
C. Elegans Model System
RAN Peptide Toxicity
Movement Analysis
Reproduction
IPTG
Carbenicillin
RNAi Feeding Clones
Hypochlorite Method
Video Speed Analysis
Movement Phenotypes
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