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Kvantificering af vævsspecifik proteostatisk tilbagegang i caenorhabditis eleganer
JoVE Journal
Biology
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JoVE Journal Biology
Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans
DOI:

09:18 min

September 07, 2021

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Chapters

  • 00:04Introduction
  • 01:09Reagent Preparation
  • 02:19Synchronization and Inhibiting Progeny Production
  • 04:05Measuring Decline in Proteostasis
  • 06:39Results: HPK-1 Promotes Proteostasis
  • 08:37Conclusion

Summary

Automatic Translation

Proteostatisk tilbagegang er kendetegnende for aldring, der letter starten af neurodegenerative sygdomme. Vi skitserer en protokol til kvantificerbart måle proteostase i to forskellige Caenorhabditis elegans væv gennem heterologe udtryk for polyglutamin gentagelser smeltet til en fluorescerende reporter. Denne model giver mulighed for hurtig in vivo genetisk analyse af proteostase.

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