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在多地址基因组集成颜色 (MAGIC) 标记的 Utero 电冲中, 以个性化皮质小鼠星形细胞
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes
Please note that all translations are automatically generated.
Click here for the English version.
在多地址基因组集成颜色 (MAGIC) 标记的 Utero 电冲中, 以个性化皮质小鼠星形细胞
DOI:
10.3791/61110-v
•
06:47 min
•
May 21, 2020
•
Laura Dumas*
1
,
Solène Clavreul*
2
,
Jason Durand
,
Edwin Hernandez-Garzon
,
Lamiae Abdeladim
,
Raphaëlle Barry-Martinet
,
Alicia Caballero-Megido
,
Emmanuel Beaurepaire
,
Gilles Bonvento
,
Jean Livet
,
Karine Loulier
1
Université de Montpellier, INSERM
,
Institut des Neurosciences de Montpellier
,
2
Sorbonne Université, INSERM, CNRS
,
Institut de la Vision
,
3
Université Paris-Saclay, CEA, CNRS, MIRCen, Laboratoire des Maladies Neurodégénératives
,
4
Laboratory for Optics and Biosciences, Ecole polytechnique, CNRS, INSERM
,
IP Paris
Chapters
00:04
Introduction
00:43
Surgical Preparation
01:39
Pregnant Mouse Preparation
02:16
Intraventricular Injection
03:16
In Utero Electroporation (IUE)
04:24
Multichannel Confocal Imaging
05:23
Results: Representative Cortical Astrocyte Segmentation and Arborization
06:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
星形细胞均匀地平铺大脑皮层,使得对其复杂形态的分析在细胞水平上具有挑战性。此处提供的协议使用基于子宫电镀的多色标签,挑出皮质星形细胞,并使用用户友好的图像分析管道分析其体积和形态。
Tags
In Utero Electroporation
MAGIC Markers
Cortical Astrocytes
Individualization
Morphology Assessment
Progenitor Cell Labeling
Neuronal And Viral Progeny
Micropipette
DNA Solution
Lateral Ventricle Injection
Embryonic Day 15.5
Surgical Procedure
Article
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