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I Utero Electroporation af Multiaddressable Genome-Integration Color (MAGIC) Markører til individualisere Cortical Mouse Astrocytes
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes
DOI:

06:47 min

May 21, 2020

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Chapters

  • 00:04Introduction
  • 00:43Surgical Preparation
  • 01:39Pregnant Mouse Preparation
  • 02:16Intraventricular Injection
  • 03:16In Utero Electroporation (IUE)
  • 04:24Multichannel Confocal Imaging
  • 05:23Results: Representative Cortical Astrocyte Segmentation and Arborization
  • 06:10Conclusion

Summary

Automatic Translation

Astrocytter flise hjernebarken ensartet, hvilket gør analysen af deres komplekse morfologi udfordrende på cellulært niveau. Protokollen her bruger flerfarvet mærkning baseret på in utero elektroporation til at fremhæve kortikale astrocytter og analysere deres volumen og morfologi med en brugervenlig billedanalysepipeline.

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