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I Utero Electroporation af Multiaddressable Genome-Integration Color (MAGIC) Markører til individualisere Cortical Mouse Astrocytes
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1Université de Montpellier, INSERM,Institut des Neurosciences de Montpellier, 2Sorbonne Université, INSERM, CNRS,Institut de la Vision, 3Université Paris-Saclay, CEA, CNRS, MIRCen, Laboratoire des Maladies Neurodégénératives, 4Laboratory for Optics and Biosciences, Ecole polytechnique, CNRS, INSERM,IP Paris
Chapters
- 00:04Introduction
- 00:43Surgical Preparation
- 01:39Pregnant Mouse Preparation
- 02:16Intraventricular Injection
- 03:16In Utero Electroporation (IUE)
- 04:24Multichannel Confocal Imaging
- 05:23Results: Representative Cortical Astrocyte Segmentation and Arborization
- 06:10Conclusion
Astrocytter flise hjernebarken ensartet, hvilket gør analysen af deres komplekse morfologi udfordrende på cellulært niveau. Protokollen her bruger flerfarvet mærkning baseret på in utero elektroporation til at fremhæve kortikale astrocytter og analysere deres volumen og morfologi med en brugervenlig billedanalysepipeline.
