JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Journal
/
Medicine
/
腱由来幹細胞の発熱分化を誘導する三次元単軸機械刺激バイオリアクターシステムの適用
JoVE Journal
Medicine
Author Produced
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Medicine
Applying a Three-dimensional Uniaxial Mechanical Stimulation Bioreactor System to Induce Tenogenic Differentiation of Tendon-Derived Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
腱由来幹細胞の発熱分化を誘導する三次元単軸機械刺激バイオリアクターシステムの適用
DOI:
10.3791/61278-v
•
14:04 min
•
August 01, 2020
•
Ziming Chen*
1
,
Peilin Chen*
1
,
Rui Ruan*
1
,
Lianzhi Chen
,
Jun Yuan
,
David Wood
,
Tao Wang
,
Ming Hao Zheng
1
Centre of Orthopaedic Translational Research, Medical School
,
University of Western Australia
Chapters
00:00
Introduction
02:33
Isolation of Mice TDSCs
03:30
Culture and Expansion of Mice TDSCs
05:12
Preparation of Stimulation Culture Medium for Cell Sheet Formation
05:44
Cell Sheet Formation by Culturing in Stimulation Medium
06:21
Preparation of 3D Tendon Stem Cell Construct
07:50
Assembly of the Uniaxial-Stretching Mechanical Stimulation Complex in Unique designed Bioreactor
09:25
Evaluation of the Mechanical Stimulated
in vitro
Tendon-like Tissue
10:10
Representative Results
11:07
Discussion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
三次元単軸的な機械的刺激バイオリアクターシステムは、腱由来幹細胞の形質特異的分化およびネオ腱形成のための理想的なバイオリアクターである。
Tags
Tendon-derived Stem Cells
Mechanical Stimulation
Bioreactor System
Tenogenic Differentiation
Tendinopathy
Cell Culture
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
人間の神経筋システムを研究するために経頭蓋磁気刺激の活用
単離、特徴付けと二つの異なる方法を使用して永久歯に由来するヒト歯髄幹細胞の分化の比較
慢性非流暢失語症脳卒中患者における言語機能を改善するために反復経頭蓋磁気刺激の活用
初代ヒト骨髄およびその悪性腫瘍を研究する三次元組織培養モデル
三次元文化:乳細胞の悪性形質転換正常対腺房アーキテクチャを研究するためのツール
3次元(3D)モデルを用いた乳癌細胞浸潤の定量化
三次元(3D)腫瘍スフェロイド浸潤アッセイ
新しい治療戦略を開発するための膵臓癌幹細胞特性を研究
ヒト下垂体腺腫細胞の三次元アルギン酸ビーズ文化
マウスで輪部幹細胞欠損を作成するための単純な機械的手順
Read Article