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Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation
JoVE Journal
Biology
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JoVE Journal Biology
Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation
DOI:

07:45 min

July 30, 2020

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Chapters

  • 00:04Introduction
  • 01:03Isolation of Porcine Cumulus-oocyte Complexes (COCs)
  • 02:35Encapsulation in Fibrin-alginate Hydrogel Beads
  • 04:48Encapsulation in Super-hydrophobic Fluorinated Ethylene Propylene (FEP) Microbioreactors
  • 06:17Results: Morphology and Viability of COCs after Encapsulation
  • 07:15Conclusion

Summary

Automatic Translation

Presented here are two protocols for the encapsulation of porcine oocytes in 3D culture conditions. In the first, cumulus-oocyte complexes (COCs) are encapsulated in fibrin-alginate beads. In the second, they are enclosed with fluorinated ethylene propylene powder particles (microbioreactors). Both systems ensure optimal conditions to maintain their 3D organization.

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