JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Chemistry
/
使用组合前体同位素标签和同位素标记 (cPILOT) 实现自动样品多路复用
JoVE Journal
Chemistry
This content is Free Access.
JoVE Journal
Chemistry
Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
Please note that all translations are automatically generated.
Click here for the English version.
使用组合前体同位素标签和同位素标记 (cPILOT) 实现自动样品多路复用
DOI:
10.3791/61342-v
•
09:24 min
•
December 18, 2020
•
Albert B. Arul
,
Renã A.S. Robinson
2,3,4
1
Department of Chemistry
,
Vanderbilt University
,
2
Vanderbilt Memory & Alzheimer’s Center
,
Vanderbilt University Medical Center
,
3
Vanderbilt Brain Institute
,
Vanderbilt University Medical Center
,
4
Vanderbilt Institute of Chemical Biology
,
Vanderbilt University Medical Center
Chapters
00:04
Introduction
00:51
Protein Extraction and Sample Preparation
03:48
Desalting Step 1
04:39
Dimethylation Labeling and Isobaric Tagging
07:21
Results: Automated cPILOT Processing of 22 Samples
08:47
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
组合前体同位素标记和同位素标记 (cPILOT) 是一种增强的样品多路复用策略,能够增加可与可用同位素标记同时分析的样本数量。机器人平台的整合大大提高了实验的吞吐量、可重复性和定量精度。
Tags
Automated CPILOT
High Throughput
Biological Information
Aging
Alzheimer’s Disease
Liver Homogenate
Urea
Denaturation Buffer
DTT
IAM
Cystine
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
定量蛋白质组学使用还原二甲基化的稳定同位素标记
适用于鼠标机关高精度锌同位素测量
定量糖组学和蛋白质组学组合式净化策略
表征,气相炭用苯聚羧酸的定量和特定化合物同位素分析(BPCA)
定点自旋标记和五聚体配体门控离子通道的EPR光谱研究
多肽和蛋白质量化使用自动免疫-MALDI (iMALDI)
利用表面有机金属化学方法,利用硅支持的催化剂进行Imine金属分析
增强QM/MM方法研究的植物双质子转移过程中的同位素效应
石英晶体微平衡测量中的蛋白质吸附和聚合物力学的样品制备
原子力显微镜与红外光谱相结合,作为探测单一细菌化学的工具
Read Article