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Generering af retinale organoider fra sunde og retinale sygdomsspecifikke human-inducerede pluripotente stamceller
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Chapters
- 00:05Introduction
- 01:19Passaging and Plating of hiPSCs to Initiate Retinal Lineage Differentiation
- 02:40Differentiation of hiPSCs into Eye Fields and Retinal Lineage
- 04:38Harvesting of Retinal Organoids
- 06:41Results: Characterization of Retinal Organoids and RPE Cells Derived From Normal and RB1-/- Mutant iPSCs
- 09:06Conclusion
Denne protokol beskriver en effektiv metode til at differentiere hiPSC'er i øjenfeltklynger og generere neuro-retinale organoider ved hjælp af forenklede dyrkningsbetingelser, der involverer både vedhængende og suspensionskultursystemer. Andre okulære celletyper, såsom RPE og hornhindepitel, kan også isoleres fra modne øjenfelter i retinale kulturer.
Tags
Keywords: Retinal OrganoidsHuman Induced Pluripotent Stem CellsRetinal DegenerationRetinal Pigment EpitheliumIn Vitro ModelsCell-based TherapiesEye DevelopmentInherited Retinal DiseasesDifferentiationAdherent CultureSuspension CultureRetinal CupsSpatial PositioningMorphological FeaturesCell DissociationIPSC CultureDifferentiation Induction MediumBFGFNoggin
