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JoVE Journal Neuroscience
Brug af primære kulturperler Hippocampal Neuroner til at studere forsamlingen af Axon indledende segmenter
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Brug af primære kulturperler Hippocampal Neuroner til at studere forsamlingen af Axon indledende segmenter

Article DOI: 10.3791/61411-v • 06:53 min • February 12th, 2021
February 12th, 2021 •
1,2,3, 2,3
1School of Basic Medical Sciences, Xi'an Jiaotong University, 2Department of Cell Biology, Duke University, 3Department of Biochemistry, Duke University

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Her beskrev vi en protokol til kvantitativt at studere samlingen og strukturen af axon indledende segmenter (AIS) af hippocampal neuroner, der mangler formonteret AIS på grund af fraværet af en kæmpe ankyrin-G.

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Tilbagetrækning Giant ankyrin-G Axon indledende segment Primær kulturperler neuroner Transfekt Imaging AIS Intensitet kvantificering
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